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Propan-2-ol esters are eluted at similar temperatures to methyl esters, so should be the favoured alternative. To show the quality of the separation that can be obtained I illustrate a separation of butyl ester derivatives of the fatty acids of aniseed oil in Figure 1. Not only sexy plump are petroselinate (6-18:1) and oleate (9-18:1) sexy plump resolved close to the base line, but cis-vaccenate also (11-18:1). Figure 1. Part GC trace of butyl ester derivatives of fatty acids from aniseed oil separated sexy plump on a CP-Sil 84 column (25 m x 0.22 mm i.d.). The temperature was held at 170°C for 3 min then was programmed at 4ºC/min to 210°C, and held at this for 15 min. Propan-2-ol and butanol will dissolve triglycerides readily so it is not necessary to add other solvents to solubilise these lipids during derivatization.
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