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Thus, for instance, the presence of 3 per cent. by weight of butyric acid in the mixed acids of butter fat really means that, out of every 100 mols. of fatty acids, about 10 mols. are butyric acid". One disavantage of area normalization is error propagation : technology the strong interdependence of results. Thus, if one fatty acid is wrongly estimated (or omitted when unknown), the results for the other fatty acids are affected. To reduce quite all kinds technology of errors, the use of an internal standard (generally C17 or C19) is recommended. If measurement uncertainty shall be technology expressed according to the ISO or EURACHEM/CITAC "Guide Quantifying Uncertainty in Analytical Measurement", the internal standard method must be adopted. The official methods of the Association of Analytical Chemists and the American Oil Chemists’s Society provide clear guidelines for accurate quantification of long-chain fatty acids.
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