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The columns were purged with nitrogen and subjected to thermal cross-linking. The resultant columns were sample preparation subjected to sample preparation extensive chromatographic testing to determine inertness, efficiency, polarity, temperature stability and suitability for the separation of fatty acid methyl esters. The columns prepared exhibited a high degree of inertness as demonstrated by isothermal and programmed Grob mix testing. Efficiencies were above 90%. Thermal studies indicated that the columns have a high upper temperature limit of 280°C. Polarity of the columns were suitable for Fatty sample preparation Acid Methyl Ester analysis as demonstrated by application chromatograms.
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