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| bacterial, plump gallery , pure emu oil, plump rumps tgp , plump fiction , alabama, plump pussies , plump latina , composition, gc packed columns, technical presentation, food information, plump tits , biochemistry and function, entomology, plump galleries , | 2002 Feb;48(2-3):259-65. Links Identification of bacteria from single colonies by fatty acid analysis.Buyer JS.US Department of Agriculture, ARS, SASL, fatty and skinny Building 001 Room 140 BARC-West, Beltsville, MD 20705-2350, USA. buyerj@ba.ars.usda.govWhile a commercially available system for microbial identification by fatty acid analysis (Microbial Identification System, MIDI, Newark, DE, USA) is available, it requires approximately 40-mg wet weight of biomass. Various authors have published fatty and skinny methods for fatty acid analysis of single colonies, but no database of organisms has been developed for those methods. A modification of the MIDI system to increase sensitivity while maintaining relative retention times and peak areas fatty and skinny allows the standard peak naming tables and libraries of organisms to be used with single colonies. Samples are evaporated down before injection to concentrate the fatty acids, while splitless injection is used to allow more sample to enter the gas chromatograph. |
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