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PB, Thompson IP, Palleroni NJ.Molecular Microbial Ecology, Institute of Virology and Environmental Microbiology, Oxford, United Kingdom.A genome agilenttechnologies and fatty acid analysis of 16 Pseudomonas stutzeri reference strains having DNA agilenttechnologies compositions ranging from 62.2 to 65.5 mol% G+C was performed by pulsed-field gel electrophoresis of agilenttechnologies XbaI and SpeI macrorestriction fragments and gas chromatography of total cellular fatty acids. Macrorestriction fragment patterns were evaluated by using previously described algorithms (D. Grothues and B. Tummler, Mol. Microbiol. 5:2763-2776, 1991), and the results allowed us to subdivide the species into two groups which correlated with G+C content. Two examples of recent strain divergence were observed among clinical isolates, but in general a marked degree of heterogeneity was observed in the macrorestriction fragment patterns, and even phenotypically similar strains produced divergent patterns.
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