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G, van der Ploeg P, Mobius M, Sawatzki G.Milupa GmbH & Co KG Research Department, Friedrichsdorf/Ts.Many different analytical procedures for fatty acid analysis of infant formulae and human milk are described. The objective was to plump your pucker study possible pitfalls in the use of different acid-catalyzed procedures compared to a base-catalyzed procedure based plump your pucker on sodium-methoxide in methanol. The influence of the different methods on the relative fatty acid composition (wt% of total fatty acids) and the total fatty acid recovery rate (expressed as % of total lipids) was studied in two experimental LCP-containing formulae and a plump your pucker human milk sample. MeOH/HCl-procedures were found to result in an incomplete transesterification of triglycerides, if an additional nonpolar solvent like toluene or hexane is not added and a water-free preparation is not guaranteed.
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