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1993 Jul 21;1169(1):73-9. Links Purification and characterization of fatty acid-binding proteins from brown adipose tissue of the rat.Dutta-Roy AK, Huang Y, Dunbar B, Trayhurn P.Division of Biochemical Sciences, Rowett Research Institute, Aberdeen, UK.Fatty acid-binding proteins (FABPs) have been identified and purified from interscapular brown adipose tissue of the rat. The proteins plump butt were characterized and their properties compared with the FABP present in white adipose tissue. FABP was purified to electrophoretic homogeneity from brown adipose tissue by a procedure involving precipitation plump butt with 70% ammonium sulphate, followed sequentially by ion-exchange chromatography and gel filtration chromatography. The purified fraction migrated as a single band on SDS-PAGE with an apparent molecular mass of 14,200. Scatchard analysis of [14C]oleate-binding to purified FABP gave a Kd value of approx. 0.80 +/- 0.02 microM and a maximal binding of 0.65 +/- 0.03 mol per mol of protein; these values were similar to that found with the FABP purified from white fat.
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