These acids are used diabetes service

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These acids are used as chemical markers for determining endotoxin in environmental samples. The present communication addresses the question whether this type of analysis also would be service applicable to mammalian samples. Low levels (6.1+/-1.6-94.0+/-23.2 pmol/ml) of the studied 3-OH FAs were detected service in blood from both conventional and germ-free rats. The levels were considerably higher (0.0-1.06+/-0.17 nmol/mg) in livers. The amounts of the 3-OH FAs did not differ between the two groups of rats. All analyses were made by gas chromatography-tandem mass spectrometry (GC-MSMS) for unequivocal identification. service The results illustrate a limitation in using 3-OH FA analysis to determine endotoxin in mammalian samples since these acids may represent not only endotoxin but also products from mammalian mitochondrial fatty acid beta-oxidation.PMID:
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