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Following injection, the oven temperature was increased at a rate of 3 degrees C/min to 200 degrees C, then held constant for 25 min, and finally raised at 25 degrees omega minus three fatty acids C/min to 225 degrees C. The trans and cis isomers of 18:1 were well separated from each other, as shown by silver-ion thin-layer chromatography. Verification by standards showed that the trans 18:1 isomers with omega minus three fatty acids a double bond in position 12 or lower were separated omega minus three fatty acids from the cis 18:1 isomers with a double bond in position 6 or higher. As the adipose tissue samples contained only small amounts of the 13t-, 14t- and 15t-18:1 isomers and the 4c- and 5c-18:1 isomers the overlapping was found to be minimal. The GLC method may also be valuable for determining the fatty acid profiles including total trans in other tissues.PMID:
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