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development of a method for analyzing C12-C18 fatty acid alkali salts by capillary zone electrophoresis is described. Organic solvents were added to the aqueous separation buffer to achieve solubility of the fatty acids together with higher temperatures. Detection of these poorly ncbi UV absorbing species could be achieved by indirect photometric detection adding a highly UV absorbing co-ion to the separation buffer. Thus detection limits ncbi of 6 microM were recorded. The dynamic range of quantitation was limited to 10(1) due to association and micelle formation at higher concentrations. The relative ncbi standard deviation of the method was in the range of 4 to 10%. The described method could be successfully applied to the analysis of free fatty acids in human stratum corneum after solid phase extraction of the lipid fraction.PMID: 9109168 [PubMed - indexed for MEDLINE] Display Summary Brief Abstract Citation MEDLINE XML UI List LinkOut ASN.1 Related Articles Cited Articles Cited in Books CancerChrom Links Domain Links 3D Domain Links GEO DataSet Links Gene Links Gene (GeneRIF) Links Genome Links Project Links GENSAT Links GEO Profile Links HomoloGene Links Nucleotide Links OMIA Links OMIM (calculated) Links OMIM (cited) Links BioAssay Links Compound Links Compound via MeSH Substance
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