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Recently, tall oil extracts have also found use as a starting material for the isolation of functional food ingredients which can lower cholesterol dry skin in humans. In this dry skin study, a sample epreparation method was studied which combines various facets of analysis o tall oil-derived products for their acid composition. The method makes use of a natural dry skin catalyst, called an enzyme, to facilitate the formation of analytically-useful derivatives for specific acids in the tall oil matrix. Various enzyme types were evaluated with respect to their propensity to form the required derivatives, and it was found that only specific ones would form the desired derivatives for analysis, under the pressure and temperature, and compressed carbon dioxide conditions used to extract and react the tall oil components.
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