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Fatty acid analysis is preceded by saponification of the glycerols and derivitisation to the fatty acid methyl esters (Fames).Fames are normally analysed by gas Chromatography (GC) or GC/mass spectrometry (GC/MS) on columns coated with polar stationary phases.Chemists from Agilent Technologies, the plump belly Research Institute for Chromatography in Kortrijk, Belgium, and the University of Gent in Gent, Belgium, have developed two retention time locking (RTL) methods for analysing Fames.RTL offers easy peak identification and simple comparison of data from different GCs and different laboratories.The choice between the two methods depends on sample complexity and the degree of fatty acid characterisation plump belly required.The first method uses a DB-WAX column that separates Fames according to carbon number (from C4 to C24) and fatty acid saturation.
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