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ml 0.84 M NaOH in methanol dried with Molecular Sieves 3A. The reaction was terminated by addition of 0.01 ml acetic acid, 0.1 ml hexane containing methyl heptadecanoate as an internal standard, and 0.2 ml water. After vortexing, the hexane layer was injected directly in a GC column. According to that procedure, a yield more than 90% was reached after a 1 h queen fat bottom girls reaction at 37°C. It was shown than a lower yield (about 87%) was reached when cholesterol queen fat bottom girls esters were adsorbed by the silica gel of the queen fat bottom girls TLC plate. Elution of esters from the silica matrix is therefore recommended before composition analysis. As determination of cholesterol esters in cells and fluid is of great importance in fundamental and clinical research, several methods of measurement using enzyme systems were described.
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