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- Analysis of free and esterified sterols in vegetal oils Verleyen T et al., JAOCS 2002, 79, 117 - A simplified method for the quantification of total cholesterol in lipids using gas chromatography Hwang BS et al., J Food Comp Anal 2003, 16, 169 100 mg of lipid are saponified, followed plump rumps by methylation with boron trifluoride. The mixture is extracted with ether, added with an internal standard (5a-cholestane) and analyzed by gas chromatography. plump rumps - Solid-phase extraction-thin layer chromatography-gas chromatography method for the plump rumps detection of hazelnut oil in olive oils by determination of esterified sterols Cercaci L et al., J Chromatogr A 2003, 985, 211 The oil was subjected to SPE, cold saponification and purification on sillica TLC. The sterol band was analyzed by direct GLC. The sterol fraction provides precise information about the origin of olive oil and a possible admixtures with hazel oil. An important review of published chromatographic methods for the analysis of plant sterols may be found in the paper by Abidi SL (J Chromatogr A 2001, 935, 173) and that by Volin P (J Chromatogr A 2001, 935, 125).
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