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plump fiction , alabama, plump pussies , plump latina , composition, gc packed columns, technical presentation, food information, plump tits , biochemistry and function, entomology, plump galleries , hplc columns, mass spectrometry, fat ass girls , and prevent cardiovascular disease, | CG, Dorland L, Holwerda redcell fatty acid analysis U, de Almeida IT, Poll-The BT, Jakobs C, Duran M.Centro de Metabolismos e Genetica, University of Lisboa, Portugal.We present a new derivatization procedure for redcell fatty acid analysis the simultaneous gas chromatographic-mass spectrometric analysis of free fatty acids and 3-hydroxyfatty acids in plasma. Derivatization of target compounds involved trifluoroacetylation of hydroxyl groups and tert-butyldimethylsilylation of the carboxyl groups. This new derivatization procedure had the advantage of allowing the complete baseline separation of free fatty acids and 3-hydroxyfatty acids while the superior gas chromatographic and mass spectrometric properties of tert-butyldimethylsilyl derivatives remained unchanged, permitting a sensitive redcell fatty acid analysis analysis of the target compounds. |
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