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| plump fiction , alabama, plump pussies , plump latina , composition, gc packed columns, technical presentation, food information, plump tits , biochemistry and function, entomology, plump galleries , hplc columns, mass spectrometry, fat ass girls , and prevent cardiovascular disease, | Run the standard. Program the sequence table to run the #68 FAME standard in first fatty acid definition available position of the ALS, the NIH reference - F control in the second position, and a hexane solvent blank in the third position. Automatic fatty acid definition calibration of retention times and response factors are automatically replaced with the calibrators data. Run the sample. Program the samples into consecutive available positions and start the sequence. Reports for each injection will be reported. Check GC Results. The lipid sample should be > 99% of the appropriate fatty acid definition fatty acid for symmetric phospholipids (e.g. |
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| Determine concentration by gravimetric analysis. Dilute to a final concentration of 20 mg/ml with additional hexane. Keep the reference standard refrigerated at 2-8°C. Stable for 6 months. Prepare the sample. Transfer mass spectrometry 5 mg of sample into mass spectrometry a test tube. If the sample is in powder form, dilute directly with 1 ml of hexane. If the sample is in mass spectrometry chloroform, evaporate the solvent with nitrogen and add 1 ml of hexane. Add 50 µl of sodium methoxide to the sample in hexane. Vortex mix. Incubate the sample at room temperature for 5 minutes. Centrifuge the sample at 1600 rpm for 5 minutes. Prepare the gas chromatograph. Instrument conditions: Injector temp. 250°C Detector temp. 220°C Oven temp. 220°C Column flow 1 ml/min Split flow 20:1 Run time 25 min. |
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