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| plump fiction , alabama, plump pussies , plump latina , composition, gc packed columns, technical presentation, food information, plump tits , biochemistry and function, entomology, plump galleries , hplc columns, mass spectrometry, fat ass girls , and prevent cardiovascular disease, | We searched for such an inhibitor in the cell. ATP-dependent vials gating of the K(ACh) channel was inhibited by the addition of the cytosolic fraction of rat atrial or brain vials tissues. The lipid component of the cytosolic fraction was found to contain the inhibitory activity. To identify the lipid inhibitor, we tested the effect of approximately vials 40 different lipid molecules. Among the lipids tested, only unsaturated free fatty acids such as oleic, linoleic, and arachidonic acids (0.2-2 microM) reversibly inhibited the ATP-dependent gating of native K(ACh) channels in atrial cells and hippocampal neurons, and of recombinant K(ACh) channels (GIRK1/4 and GIRK1/2) expressed in oocytes. Unsaturated free fatty acids also inhibited phosphatidylinositol-4, 5-bisphosphate (PIP(2))-induced changes in K(ACh) channel kinetics but were ineffective against ATP-activated background K(1) channels and PIP(2)-activated K(ATP) channels. |
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| 2000 Mar;115(3):287-304. Links Cytoplasmic unsaturated free fatty acids technical presentation inhibit ATP-dependent gating of the G protein-gated K(+) channel.Kim D, Pleumsamran A.Department of Physiology, Finch University of Health Sciences, The Chicago Medical School, technical presentation North Chicago, Illinois 60064, USA. kimd@mis.finchcms.eduThis study reports the identification of an endogenous inhibitor of the G protein-gated (K(ACh)) channel and its effect on the K(ACh) channel kinetics. In the presence of acetylcholine in the pipette, K(ACh) channels in inside-out atrial patches were activated by applying GTP to the cytoplasmic side of the membrane. In these patches, addition of physiological concentration of intracellular ATP (4 mM) upregulated K(ACh) channel activity approximately fivefold and induced long-lived openings. However, such ATP-dependent gating is normally not observed in cell-attached patches, indicating that an endogenous substance that inhibits the ATP effect is present in the cell. |
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