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plump fiction , alabama, plump pussies , plump latina , composition, gc packed columns, technical presentation, food information, plump tits , biochemistry and function, entomology, plump galleries , hplc columns, mass spectrometry, fat ass girls , and prevent cardiovascular disease, | The simplified method is rapid, easy-to-use, suitable research association for analysis of total long chain polyunsaturated fatty acid contents (e.g. research association n-6 and n-3 fatty acids) in various biological samples, especially when the number of samples to be analyzed is large and/or the specimen size is small. Background Fatty acid composition of cell membrane is an important determinant of cell function [1]. Manipulation of cellular fatty acid composition has been a widely used approach to modulating the biological responsiveness of different cell types. Recently, fatty acid profile, particularly the ratio of omega-6 (n-6) to omega-3 (n-3) polyunsaturated research association fatty acids, of cells or tissues has become a biomarker for monitoring the outcome of dietary interventions (i.e., fatty acid supplementation) and for identifying the risk factors for lipid related diseases (e.g. |
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This conventional method is complicated, tedious and time consuming. Development of a rapid and simple method for lipid analysis is warranted. Results We simplified the conventional method by combining the extraction and methylation into a single step (omitting and prevent cardiovascular disease the procedure of prior extraction). Various biological samples including cultured cells, animal tissues and human specimens have been tested using the new method. Statistical analysis indicates that the recovery of long chain and prevent cardiovascular disease fatty acids from tissue samples by the simplified method is significantly higher than that by the traditional method, but there is no difference in relative fatty acid and prevent cardiovascular disease composition between the two methods. This simplified method can significantly save time and materials, and reduce the potentials of sample loss and contamination. Conclusion The lipid extraction procedure prior to methylation employed conventionally in lipid analysis can be omitted without affecting the recovery of long chain (≥ 18 C) fatty acids and their composition. |
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