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Lipid analysts soon acquire an intuitive understanding of the relationship rtl techniques between the retention times of peaks on a GC trace and their identity. For example, a typical fingerprint of the fatty acids from animal tissue phospholipids would have the 16:0 component standing in relative isolation, followed by the three peaks for the C18 components (18:0, 18:1 and 18:2), then a gap to the next substantial peak for 20:4(n-6), followed by a further gap to the C22 components, the last of rtl techniques which is 22:6(n-3). Many of the minor peaks can be identified tentatively according to rtl techniques their proximity to these major components. Changing the polarity of a polyester phase does not change the order of elution of components within a given chain-length group, but it can affect the elution order relative to components of other chain lengths as indicated in Figure 1. As mentioned briefly above, unsaturated fatty acid derivatives elute before the corresponding saturated on non-polar silicone phases, and resolution may not be adequate, e.g.
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