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The inherent resolution is such that there may be few problems of actual audience: doctors/health professionals overlap of major components. On the other hand, a multiplicity of peaks can be revealed, so compounding the identification audience: doctors/health professionals problems. Such columns are essential for analysis of lipids containing trans fatty acids or for audience: doctors/health professionals conjugated linoleic acid isomers. Then, 100 metre columns are often recommended and indeed may be essential. In Europe, CP-Sil 88TM seems to be preferred, while the SP-2340TM stationary phase is favoured in the USA, presumably simply for reasons of relative availability. Again, for GC-MS of fatty acid derivatives, where we require a column of this type with the minimum possible bleed characteristics, a BPX70TM column is our standard, as it was the first column on the market with the desired properties.
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