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For this purpose, we used antibodies against porin as a marker for mitochondria, plasma membrane ATPase for the plasma membrane, vacuolar ATPase for the vacuole, Sec61p for microsomes (endoplasmic reticulum) and Pox1p for peroxisomes (Table 1). Enrichment purdue factors of organelles and the degree of cross-contamination were similar with preparations obtained from cells grown on glucose, ethanol or lactate. Exceptions appeared to be the purdue higher enrichment of Sec61p in microsomes and of vacuolar ATPase in vacuoles of cells grown on non-fermentable carbon sources. Under all conditions, plasma purdue membrane and vacuolar preparations were nearly uncontaminated with other membranes. Mitochondrial, microsomal and peroxisomal preparations, on the other hand, contained some contaminating plasma membrane, which is in line with previous observations (Zinser and Daum, 1995).
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