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sexy plump women , market intelligence, qfasa, plump amateur , plump women galleries , agency for healthcare research and quality, retention, fatty vautin , links, plump granny , information, dress fatty , cardiovascular disease, fat naked girls , | 1998). Sterols The plasma membrane of S. cerevisiae is the fraction with the highest concentration of free sterols (Zinser et al., 1993). The most prominent sterol in the yeast plasma membrane is ergosterol (90-98% of total sterols), and sterol precursors can be detected only at minor amounts omega3 fatty acid (Tables 5, 6 and 7). A similar sterol composition was determined for peroxisomal membranes. The sterol concentration in omega3 fatty acid other yeast subcellular membranes omega3 fatty acid analyzed during this study, namely mitochondria, microsomes and vacuoles, is rather low (Zinser et al., 1993). These organelles, however, contain a higher portion of precursor sterols than the plasma membrane (see Tables 5, 6 and 7). |
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It has to be taken into account, however, that enrichment factors of 5-7 for plasma membrane qfasa ATPase in mitochondria, microsomes and peroxisomes versus an enrichment factor of 100-120 of the marker in the plasma membrane itself is indicative of a comparably low degree of contamination of the former fractions. Phospholipids qfasa Major phospholipids of mitochondria, microsomes, vacuoles and peroxisomes are phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol (Tables 2, 3 and 4). This lipid composition was observed regardless of qfasa the carbon source used for cell cultivation. In the plasma membrane, phosphatidylserine is the major phospholipid. This enrichment was found at the highest level with cells grown on glucose. In mitochondria, the amount of cardiolipin, a mitochondria specific phospholipid (Daum, 1985), was increased to a great extent when cells were cultivated on non-fermentable carbon sources thus confirming previous results from our laboratory (Tuller et al., |
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