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| plump wife , plump mt , essential fatty acids in human nutrition, plump blonde , pictures of fat girls , laboratories, big and plump , food science, trans, big plump plumpers galleries com , plump babes , eulachon, plump moms , symptoms of fatty liver , auke bay lab, liens, fat girls in thongs , fattyacids, plump dumpling , mutant, seabird, plump furries , pharmaceuticals, | Here, we present a new method using a modified Jones' reagent to oxidize these alcohols and DMAs to free fatty acids (FFAs). These FFAs are then easily methylated and quantitatively recombined with FAMEs from the same sample. This generates a fatty acid nutrition research signature of prey that is equivalent to that which the predator has available for deposition upon digestion of that prey. This method is validated with alcohol and DMA standards. Its application nutrition research to typical marine samples is also presented, demonstrating the change in effective fatty acid signature after inclusion of nutrition research fatty acids derived from wax esters and plasmalogens.PMID: |
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| 2003 Sep;44(9):1802-7. Epub 2003 Jun 16. Links Quantitative analysis of fatty acid precursors in marine samples: direct conversion of wax ester alcohols and dimethylacetals to FAMEs.Budge SM, Iverson SJ.Department of Biology, big and plump Dalhousie University, 1555 Oxford Street, Halifax, Nova Scotia, Canada. budges@dal.caTo apply fatty acid analyses to the big and plump study of foraging ecology and diet determination, all compounds that may be deposited as fatty acids in a big and plump predator must be quantified in the prey. These compounds include the usual fatty acids in acyl lipids, but also the alcohols of wax esters and the vinyl ethers of plasmalogens. In routine fatty acid analysis, samples are extracted and transesterified (methylated), resulting in the formation of fatty acid methyl esters (FAMEs); however, fatty alcohols and dimethylacetals (DMAs) are also generated if wax esters or plasmalogens are present. |
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