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1983 Feb;24(2):216-20. Links Hydrazinolysis of lipids and technology analysis of their constituent fatty acids.Agrawal VP.Treatment of O-acyl lipids with hydrazine degrades them to hydrazides of the constituent fatty acids. These hydrazides react with acetone to yield less polar and more volatile N'-isopropylidenealkanohydrazides, R-CO-NH-N = C(CH3)2, which strongly absorb ultraviolet light and are amenable to thin-layer and gas-liquid chromatographic analyses. In presence of cyclopentene, technology reduction of double bonds in unsaturated fatty acids with hydrazine does not occur. The method of hydrazinolysis followed by acetonization has been used for technology fatty acid analysis of different classes of lipids.PMID:
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