mm I.D.). Peaks were audience: medical/academic researchers plump mature

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mm I.D.). Peaks were identified by comparison with fatty acid standards (Nu-chek-Prep, plump mature Elysian, MN), and area and its percentage for each resolved peak were analyzed using a Perkin-Elmer M1 integrator. Simplified method An aliquot of cell pellet or tissue homogenate (<50 μl) in a glass methylation tube was mixed with 1 ml of hexane and 1 ml of 14% BF3/MeOH reagent. After blanketed with nitrogen, the mixture was heated at 100°C for 1 hour, cooled to plump mature room temperature and methyl esters extracted in the hexane phase plump mature following addition of 1 ml H2O. The samples were centrifuged for 1 minute, and then the upper hexane layer was removed and concentrated under nitrogen. Fatty acid methyl esters were analyzed by gas chromatography as described above. Statistical analysis The results from the two methods were compared by using unpaired t-test, and P values of <0.05
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