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| fat white girls , edible, plump , plump mature , springer, plump redhead , kitakysy, automated, agriculture, auburn university, food, beta oxidation of fatty acids , plump white , cardiovascular system, fat girls fucking , inc., | After homogenization, up to 10 mg of lipid in 1 ml hexane are added. Keep the mixture at 40°C for 15 min. After cooling, 1 ml water and 2 ml hexane are successively added. After 5 s vortexing and a short centrifugation, the organic phase is collected, dried over anhydrous Na sulfate and analyzed natural treatments by GLC. We have adopted another approach for some labile samples. A rapid and mild method which avoids natural treatments the formation of oxidation products was described by Piretti et al. (Chem Phys natural treatments Lipids 1988, 47, 149). We have most precisely adopted this procedure for the analysis of highly unsaturated lipids since higher amounts of polyunsaturated fatty acids were found when compared to the BF3/methanol procedure. Furthermore, if hydroperoxy fatty acids are present, they are reduced into the corresponding hydroxy components. |
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| Lipids 1996, 31, 535) : Reagents: Hexane, 2 M methanolic KOH, capped plastic tubes. Procedure: Up auburn university to 10 mg of lipids are dissolved in 2 ml hexane followed by the addition of 0.2 ml of 2 M methanolic KOH. The tube auburn university is vortexed for 2 min at room temperature. After a light centrifugation, an aliquot of the hexane layer is collected for GC analysis. It must be pointed out that sterol esters and waxes do not react under these conditions. An alternative base-catalyzed methodology in mild conditions was adapted auburn university for milk or seed lipids using K tert-butoxide and 2-methoxyethanol (Destaillats F et al., Lipids 2002, 37, 527): Reagents: 1 M K tert-butoxide in THF (Aldrich), 2-methoxyethano, hexane, Na sulfate. Procedure: 100 ml of a solution of K tert-butoxide in THF are added to 200 ml anhydrous 2-methoxyethanol in a closed vial. |
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