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Reagent 14% Boron trifluoride in methanol (Alltech or Sigma) (keep refrigerated under nitrogen and discard after 3 months or when solids appear at the bottom of fat ass white girls the vial). Pentane, fat ass white girls chloroform. Procedure As a general procedure, an aliquot of lipid extract (about 10 mg) is dried under nitrogen in a screw-capped glass tube and 1 ml of BF3/methanol is added. If triacylglycerols or sterol esters fat ass white girls are analyzed alone or are abundant in the extract, the dry lipids are dissolved in 0.75 ml of chloroform/methanol (1/1, v/v) and 0.25 ml BF3/methanol are added. If possible, the tube is closed after flushing with nitrogen. Heat in boiling water (or at 100°C in a sand bath) the time indicated for the respective lipid: Lipids Heating time (min) Fatty acids 5 Triacylglycerols 45 Sterol esters 45 Monoacylglycerols 15 Diacylglycerols 15 Glycerophospholipids 15 Glyceroglycolipids 15 Sphingomyelin 90 Glycosphingolipids 90 After cooling, add 1 ml water and 2 ml pentane.
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