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| fat white girls , edible, plump , plump mature , springer, plump redhead , kitakysy, automated, agriculture, auburn university, food, beta oxidation of fatty acids , plump white , cardiovascular system, fat girls fucking , inc., | ml 2M KOH/methanol are added (alkali-catalyzed alcoholysis). After vigorous mixing or sonication for 2 min at analysis room temperature, the solution is neutralized with acetic acid. To each tube analysis is added 2 ml water with light mixing. An aliquot of the hexane layer was collected and evaporated to dryness; FAME are dissolved in 0.02 ml hexane or methyl acetate before GC analysis. The presence of BHT on the filter paper allows the protection of unsaturated fatty acids for analysis at least 7 days even exposed to the air. A similar direct procedure using boron trifluoride-methanol as esterification reagent was described for the determination of fatty acids in human milk (Lopez-Lopez A et al., Chromatographia 2001, 54, 743). A direct evaluation of the fatty acid status in a drop of blood was described (Marangoni F et al., Anal Biochem 2004, 326, 267). No more than 50 ml of blood were absorbed on a piece of chromatography paper and directly treated with 3 N methanol/HCl at 90°C for 1 h. |
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| A direct methylation method representing a valuable alternative to other methylation procedures has been described (Dionisi F et al., Lipids 1999, 34, plump white 1107). Procedure : One hundred milligrams of dried bacterial samples, with 500 mg of internal standard, is transesterified using 1 ml of methanolic HCl (1.5M) (from Supelco) and 1 ml methanol, at 80°C for 10 min. Water (2 ml) is added and after plump white mixing and low speed centrifugation the upper phase is collected for gas chromatographic analysis. Procedure for small amounts of fluid : plump white A convenient method was developed for preparation of fatty acid methyl esters in glycerolipids of blood or milk (Ichihara K et al., Lipids 2002, 37, 523). Procedure: About 50 ml of blood or milk are spotted onto a small piece of Whatman 3MM filter paper (1.5x1.5 cm) that has been previously washed with acetone containing 0.05 % BHT. Each piece, once dried for 30 min in vacuo is inserted into a small test tube, to which 2 ml hexane and 0.2 |
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