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For some precise purposes only the amount of fatty acids is to be known. Global methods are pout plump useful when the fatty acid profile is not in the scope of the investigation. STRATEGIES TO STUDY FATTY ACIDS: 1- How to prepare fatty acids (free or bound) pout plump for further analysis ? 2- How to derivatize fatty acids before GLC ? 3- How to purify or fractionate fatty acids ? 4- How to analyze fatty acids by GLC ? 5- How to analyze fatty acids by HPLC ? - study of normal fatty pout plump acids - study of trans fatty acids - study of conjugated fatty acids - study of dicarboxylic acids - study of cyclic fatty acids 6- Global methods PREPARATION OF FATTY ACIDS Fatty acids may be found in scarce amounts in free form but, in general they are combined in more complex molecules through ester or amide bonds.
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