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J High Resol Chromatogr 1994, 17, 662). This method was also used to fatty acid ester isolate fatty acid ethyl esters from alcoholic beverages. Shortly, the SPE cartridges are prepared in washing with methanol and water. 50 ml of liquid are passed through the column followed by a washing with acidified water. Analytes are eluted with 2 ml dichloromethane and 2.5 ml pentane. The extraction of long-chain fatty acids fatty acid ester from fermentation medium and industrial effluents with a 98 to 100% recovery was described (Lalman JA et al., JAOCS 2004, 81, 105). Maximal recovery was obtained by adding 2 ml fatty acid ester of hexane/ter-butyl methyl ether (1/1), 80 ml of 50% H2SO4, and 0.05 g NaCl to 1 ml of the aqueous sample and mixing for 15 min at 200 rpm. A lower recovery was obtained only for caproic (C6:0) and caprylic (C8:0) acids : 27 and 76% recoveries, respectively. The purification of free fatty acids has been done by solid-phase microextraction (SPME) (Tomaino RM et al.
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