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| fat white girls , edible, plump , plump mature , springer, plump redhead , kitakysy, automated, agriculture, auburn university, food, beta oxidation of fatty acids , plump white , cardiovascular system, fat girls fucking , inc., | Procedure for small tissue samples : A tissue sample containing as low as 10 mg of lipids is introduced at the bottom of a screw-capped tube (Teflon-lined). Then add omega3 fatty acids 1 ml of methanolic HCl, 1 ml of methanol and 0.5 ml hexane. Close tightly the omega3 fatty acids tube and heat at 100°C for 1 h (shake several times). After cooling add 2 ml of hexane and 2 ml of water. Mix not too vigorously the tube and collect the hexane layer after a short centrifugation. Before GC analysis, the extract may be concentrated by evaporation under nitrogen if necessary. In omega3 fatty acids lipid-producing bacteria or microheterotrophs, the direct transesterification method was shown to be the most efficient to study the fatty acid profiles (Lewis T et al. |
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| Reagents: 2M NaOH, NaBH4, anhydrous Na2SO4. Ethyl acetate, methanol, hexane. Procedure: 2 mg of neutral lipids or up to 100 mg polar lipids are dried in a glass tube. Add 1 ml of the reagent made in food dissolving immediately before use 400 mg NaBH4 in 10 ml of the mixture methanol/2 M NaOH (19/1, v/v). The mixture is stirred for 20 min at room temperature. After adding 2 ml water, food the methanol is eliminated under nitrogen. The methyl esters are recovered from the aqueous phase by extracting 3 times with 1 ml ethyl acetate. food The organic phase is then washed 3 times with 1 ml water and dried by adding Na2SO4. After vortexing and centrifugation, the ethyl acetate is evaporated and the residue dissolved in a small amount of hexane for GLC analysis. C - Direct transmethylation without prior extraction The concept of direct transesterification of techniques has been reported for small tissue samples (1-10 mg) or small volumes (about 50 ml) of biological fluids (blood, milk). |
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