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Combined basic and acid hydrolysis Another practical approach to the technical problem of the hydrolysis of sphingolipids has been described using a one-spot gc accessories heating in a microwave oven with 0.1 M NaOH in methanol for 2 min followed by 1M HCl in methanol for 45 s (Itonori S et al., J Lipid Res 2004, 45, 574). DERIVATIZATION BEFORE gc accessories GLC gc accessories Before GLC analysis it is necessary to prepare non-reactive derivatives of fatty acids (methyl esters or other derivatives) which are also more volatile than the free acid components. Acylated lipids are transformed by a transesterification reaction by which the glycerol moiety is displaced by another alcohol (methanol, butanol, ...) in acidic conditions (HCl or BF3). The generation of methyl esters can be done in acidic or in alkaline conditions on isolated lipids or fatty acids but also directly by a one-step procedure combining lipid extraction and transesterification on small amounts of dried tissue.
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