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Several directions are suggested acid for development of improved methods. There can be little doubt that gas chromatography is the only technique that need be considered for routine analysis of most fatty acid samples. The flame-ionization detector is robust and has an enormous dynamic range, so accurate quantification is rarely a problem. Therefore, is there any place for high-performance liquid chromatography (HPLC) for the analysis of fatty acids? The answer is an undoubted yes, perhaps not for mainstream samples but certainly for the less usual. A major advantage can be that acid HPLC operates acid at ambient temperature so there is relatively little risk to sensitive functional groups. It should also be remembered that HPLC is not merely an analytical technique, but can be used equally easily for micro-preparative purposes.
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