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This appears to be because acetonitrile interacts in a specific way with the p electrons of double bonds. Methanol does not interact with double bonds in the same way, and choiceof gc columns for fatty acid analysis it seems that separations of fatty choiceof gc columns for fatty acid analysis acid derivatives are less affected by the nature of the gradient when this is used. There have been attempts to use ternary mixtures of methanol-acetonitrile-water or acetonitrile-tetrahydrofuran-water, but none appear to offer special advantages. Note added later: I have recently shown that it is possible to use acetonitrile alone as the choiceof gc columns for fatty acid analysis mobile phase, but with a flow gradient, and this simplifies the isolation of specific components in micro-preparative HPLC, although it may reduce the selectivity of the separation (Christie, W.W. Lipids, 33, 343-353 (1998)). With the correct choice of stationary phase, it is possible to separate picolinyl ester and DMOX derivatives of fatty acids).
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